How to conduct protein expression in bacteria?

I learned that the first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins.

Can anyone experienced in this field explain in details?

Asked Sep 09, 2015

TIP: If it's not your answer to this question, please click "Leave a Comment" button under the question to communicate with the question owner.