Biochem help asap plz

If you chromatograph an oblong protein, why does it appear to be larger than a spherical protein of equal mass?

Explain how you prepare an 80mL column of an appropriate sephadex gel needed to separate proteins ranging in size from bovine serum albumin to beta-amylase?

How can you use the equation for kd and data to calculate the exclusion limit for sephadex G-75?
Asked Mar 11, 2014

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