In my opinion... In a business situation, no. If they are all relatives, just do it for generations older than your own.
Dr. Manners says:
http://en.allexperts.com/q/Social-Etiquette-Good-2570/pushing-lady-chair.htm
It's tricky because some women don't like it, and others think a man is a barbarian if he doesn't. It's helpful if you know the ladies well enough to know their preferences. I prefer that my husband not pull out my chair because he's usually more tired than I am in the evenings and I feel like I should be pulling out a cot for him instead.